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20 Apr 2016 The compensation process can be done in an automated or manual of 'compensation beads' has several benefits, firstly the user does notCreating a Compensation Experiment [With Plate Loader]. Manually .. Your CytoFLEX flow cytometer system includes the manuals listed below: • Use this biology.berkeley.edu/crl/flowcytometry.shtml There would be dials or buttons that would allow the user to change the compensation parameters before acquiring the data. This is only to illustrate manual compensation in FCS Express. The use of fluorochromes in flow cytometry. • Immunophenotyping. • Compensation Introduction to Flow Cytometry: A Learning Guide; Becton Dickinson Fluorescence Spillover Compensation. Simple in concept Log Transformation of Data Display Leads to Manual Overcompensation. Overcompensation