METHODS GRADING: Full grading was done in accordance with the line of the paper at 6 minutes to the graph centre point at 6 minutes. This figure is an indication of Vicam Aflatest Instruction Manual May 5, 1999 Vicam DON FQ Instruction Manual June 11, 2002 Aflatest fluorometer (VICAM fluorometer Series 4) was used for estimation of the incidence of contamination of nuts with AFT according to the method described in the instruction manual of the producing company (Vicam Aflatest instruction manual method No.4.19) (Truckess et al., 1991). The fluorometer was first calibrated with aflatoxins The Aflatest® agent used in the development was added to the eluate and aflatoxin content was read with a fluorimeter (series 4, Vicam). Detection limit of the method was 1 µg/kg. 2.4.2. Ochratoxins (Ochratest Manual, 2002) For analysis of ochratoxins, 50 g of each sample were homogenized in a blender with 100ml methanol:water (80:20) for one (In house VICAM Aflatest Instruction Manual, 2014, p. 36) Immuno affinity Column /HPLC (In house VICAM Aflatest Instruction Manual, 2014, p. 11) Immunoaffinity Column /HPLC(ln house VICAM Aflatest Instruction Manual, 1999, p.15 -16) 400 400 400 400 ,200 1,200 650 1 1 FLU. 2559 . 41 45 2560 The aim of this work was to investigate influence of commercial baker's yeast in two different concentrations on the production of the AFB1 by three isolates of Aspergillus flavus, that showed ability to produce AFB1 in high concentrations. Samples were analyzed by VICAM's AflaTest. Aflatoxin Contamination of Selected Staple Foods Sold for Human Consumption in Kampala Markets, Uganda Total aflatoxins were identified and quantitated using the VICAM AflaTest fluorometric method (VICAM LP, 34 Maple Street, Milford, MA 01757 USA). Samples of sorghum grain and malt, traditional opaque sweet beverage (thobwa) and beer prepared from sorghum malts, were collected from the southern region of Malawi during the humid month of January.The samples were an