6 Combined Radioactive and Non-Radioactive Double In Situ Hybridization (DISH) Jong-Hyun Son and Ursula H. Winzer-Serhan 7 One FISH, dFISH, three FISH: Sensitive Methods of Whole-Mount Fluorescent In Situ Hybridization in Freshwater Planarians. David D. R. Brown and Bret J. Pearson A non-radioactive in situ hybridization technique has several advantages with respect to the radioactive technique, including the long half life of the probes (no loss of activity in one year storage at ?20°C), the higher spatial resolution, the shorter duration of each experiment (24-48 h) and does not need any special safety measures or ! 1! Non Radioactive in situ Hybridization with Arabidopsis Floral Tissue Kay Schneitz TUM, Entwicklungsbiologie der Pflanzen, Emil-Ramann-Str. 4, 85354 Freising, Deutschland. Modified and updated by Maxi Oelschner. Non Radioactive in situ Hybridization with Arabidopsis Floral Tissue S. Balasubramanian, D. Chevalier, P. Sieber and Kay Schneitz. Entwicklungsbiologie der Pflanzen, Technische Universitat Munchen, Hochanger 4, 85354 Freising, Deutschland. The protocol is based on protocols of G. Drews, J. Long, K. Barton and U. Grossniklauss. PDF | Nucleic acid hybridization techniques have contributed significantly to the understanding of gene organization, regulation and expression. In situ hybridization is a method for detecting sectioned and directly subjected to in situ hybridization without the ?nal step of uranyl acetate staining. In situ hybridization was performed according to the non-radioactive in situ hybridization application manual (Boehringer Mannheim). Brie?y, ultra-thin sections were ?rst treated with proteinase K (10 µg/ml) for 15 minutes at 37°C. Assessment of decalcifying protocols for detection of specific RNA by non-radioactive in situ hybridization in calcified tissues Accepted: 3 January 2000 transmission of soft X-ray. After decalcification was complete, the In situ hybridisation: radioactive vs. non-radioactive. Whi