Circular Dichroism Spectroscopy—Circular dichroism spectra were acquired on a Jasco J-715 spectrophotometer. Spectra were recorded from 185 to 240 nm using 30 scans, a scanning speed of 200 nm min-1, and a 1-nm bandwidth. Jasco J-710 CD Summary. The Jasco J-710 CD is a research grade instrument used for measuring the circular dichroism of compounds in solution, including small molecules, peptides and proteins. The instrument was purchased in 1995 and upgraded in 2005 to a J-715 instrument, the highest level of firmware and software available for this series. Jasco Cd-2095 Plus Chiral Detector Circular Dichroism Polarimeter For Hplc . Lot Of - $1,899.00. Lot Of 7 - Zeiss Gdx Vcc Scanning Laser Polarimeter. Automatic Polarimeter - $2,089.01. Automatic Polarimeter +-45 Sodium Lamp Wcell Lcd Display Wzz-2s. Circular dichroism (CD) is an excellent method for rapidly evaluating the secondary structure, folding and binding properties of proteins. Briefly, circular dichroism is defined as the unequal absorption of left-handed and right-handed circularly polarized light. A beam of light has time dependent electric and magnetic fields associated with it. Jasco Spectropolarimeter J-715. High-precision instrument for CD measurements (manual). A powerful xenon lamp combined with a double monochromator enables accurate wavelength selection in the 165 to 900 nm range. The CD apparatus contains a photo elastic modulator (PEM) that generates circularly polarized light. In addition to SRCD measurements, a regular CD spectrometer was used to obtain a melting curve for P2. Briefly, the CD signal was followed at 218 nm as a function of temperature (+20 to +95°C, slope +1°C/min) in a Jasco J-715 Spectropolarimeter. The protein concentration was 0.12 mg/ml and a 1-mm quartz cuvette was used. CD spectra were recorded on a JASCO J-715 F. Trajectory Analysis spectropolarimeter; model J-715 (Japan) equipped with a The radius of gyration (Rg) and root mean square deviation temperature controller using quartz cells (1 mm, 10mm). Find great deals on eBay for polarimeter. Shop with confidence. All samples were prepared at a protein concentration of 3 ?M (protomer concentration) in a buffer containing 100 mM potassium phosphate, pH 7.4. CD spectra were recorded at 25 °C with a Jasco J-715 spectropolarimeter equipped with a Peltier temperature control unit (Jasco Easton, MD, USA). Perkin Elmer Uv Winlab Manual CD spectrum was recorded for the GFP lead sensing variant on a Jasco J-715. Instruments/Software used: PerkinElmer Lambda 35 UV-vis Spectrometer UV WinLab, Version 2.85.04, 236 pages Chem 4190 SpectroscopySolution Manual.